Optimization of Conditions of Determination of Selenium in Blood Serum by Atomic Absorption Spectrometry

Autoři

  • D. Milde Department of Analytical Chemistry, Faculty of Science, Palacky University, Tr. Svobody 8, 771 46 Olomouc,
  • M. Sobrova Department of Analytical Chemistry, Faculty of Science, Palacky University, Tr. Svobody 8, 771 46 Olomouc,
  • J. Machacek Oncological Clinics, Faculty Hospital Olomouc, I.P. Pavlova 6, 775 20 Olomouc
  • V. Stuzka Department of Analytical Chemistry, Faculty of Science, Palacky University, Tr. Svobody 8, 771 46 Olomouc,

Abstrakt

Methods of determination of selenium in samples of dilute and mineralized blood serum by AAS with electrothermal atomization and hydride generation are described. Use of various matrix modifiers (Pd(NO3)2 + Mg(NO3)2, Ni(NO3)2 + Mg(NO3)2 and Pd + ascorbic acid) and introduction of air into a cuvette during pyrolysis are discussed. With hydride generation, the influence of interfering substances (sodium and HNO3 residues after mineralizations) and optimization of the quartz atomization tube temperature are described. For all procedures, the limits of detection, determination and repeatability as well as the results of analysis of a reference material (SeronormTM Trace Element Serum) and testing of reversibility are given.

Stahování

Publikováno

15.09.2002

Jak citovat

Milde, D., Sobrova, M., Machacek, J., & Stuzka, V. (2002). Optimization of Conditions of Determination of Selenium in Blood Serum by Atomic Absorption Spectrometry. Chemické Listy, 96(8). Získáno z http://ww.w.chemicke-listy.cz/ojs3/index.php/chemicke-listy/article/view/2310

Číslo

Vol 96 No 8 (2002)

Sekce

Články