Aplikace metody LAMP pro detekci nežádoucích bakterií v syrovátce

Autoři

  • Eva Šviráková Ústav konzervace potravin, Fakulta potravinářské a biochemické technologie, Vysoká škola chemicko-technologická v Praze, Praha, Česká republika https://orcid.org/0000-0001-7090-0117
  • Kateřina Loupancová Ústav konzervace potravin, Fakulta potravinářské a biochemické technologie, Vysoká škola chemicko-technologická v Praze, Praha, Česká republika
  • Irena Němečková Výzkumný ústav mlékárenský s.r.o., Praha, Česká republika https://orcid.org/0000-0002-7499-8964

DOI:

https://doi.org/10.54779/chl20220693

Klíčová slova:

metoda LAMP, detekce bakterií, nežádoucí bakterie, syrovátka

Abstrakt

The LAMP method (Loop-Mediated Isothermal Amplification) is a molecular biological method for the amplification of specific DNA and RNA sequences suitable for the detection of various pathogenic, semi-pathogenic and technological undesirable microorganisms. The aim of the work is using the LAMP method for the option verification of detection of health and technologically undesirable bacteria in liquid sweet whey under laboratory conditions. Experiments were carried out with 12 bacterial strains, including three representatives of Gram-positive bacteria (Enterococcus spp., Streptococcus sp.) and 9 representatives of Gram-negative bacteria (E. coli, Kl. pneumoniae, Ps. aeruginosa). The Eazyplex® BloodScreen GP/GN Assay kits (AmplexDiagnostic GmbH, Germany), designed for Gram-positive (GP) and Gram-negative (GN) bacteria and commonly used in clinical laboratory practice, were used for the LAMP experiments. Based on the obtained results it was concluded that the LAMP method was suitable for the detection of tested bacteria at the level of the species in liquid sweet whey. The results of this work can be used in the application of the LAMP method in dairy laboratory practice, in the targeted assurance of health safety and quality of food raw materials and products, especially liquids.

Stahování

Publikováno

15.11.2022

Jak citovat

Šviráková, E., Loupancová, K., & Němečková, I. (2022). Aplikace metody LAMP pro detekci nežádoucích bakterií v syrovátce. Chemické Listy, 116(11), 693–699. https://doi.org/10.54779/chl20220693

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