Techniky metabolomiky v biomedicíně
Klíčová slova:metabolomika, plynová chromatografie, kapalinová chromatografie, kapilární elektroforéza, hmotnostní spektrometrie, zpracování dat
Global metabolite profiling, often called metabolomics, is an expanding research field. Together with genomics, transcriptomics and proteomics, it provides additional information on specific changes occurring in biological systems, allowing to better understand metabolic pathways and their pathologies. This review highlights the current metabolomic challenges in biomedicinal research using chromatographic and electrophoretic separation techniques coupled mostly with mass spectrometry. The most frequently used procedures for obtaining bioinformation data are introduced as well.