The Technology of Digital PCR for Analysis of The Deletion Polymorphism in the GSTM1 Gene

Authors

  • M. Beránek Institute of Clinical Biochemistry and Diagnostics, Faculty of Medicine, Charles University and Faculty Hospital Hradec Kralove, Hradec Králové | Department of Biochemical Sciences, Faculty of Pharmacy in Hradec Kralove, Hradec Králové
  • Z. Fiala Institute of Hygiene and Preventive Medicine, Faculty of Medicine in Hradec Kralove, Charles University in Prague, Hradec Králové
  • J. Kremláček Institute of Pathological Physiology, Faculty of Medicine in Hradec Kralove, Charles University in Prague, Hradec Králové
  • V. Palička Institute of Clinical Biochemistry and Diagnostics, Faculty of Medicine, Charles University and Faculty Hospital Hradec Kralove, Hradec Králové
  • L. Borská Institute of Pathological Physiology, Faculty of Medicine in Hradec Kralove, Charles University in Prague, Hradec Králové

Keywords:

digital PCR, GSTM1, polymorphism, genotoxicity, deletion, null allele

Abstract

Glutathione S-transferase M1 is a cytosolic enzyme important for the biotransformation of xenobiotics in various human tissues. The aim of this study was to perform genetic analysis of deletion polymorphism in the GSTM1 gene by using the technology of digital PCR. For genotyping, the QX100 Droplet Digital PCR System was used. The absolute quantity of GSTM1 copies was normalized to the β-globin reference gene. In our experimental group, the prevalence of GSTM1*0 null variant was 67 %. Frequencies of GSTM1*1/*1 (n=5), GSTM1*0/*1 (n=23), and GSTM1*0/*0 (n=22) genotypes were 10 %, 46 %, and 44 %, respectively. Digital PCR seems to be an available and reliable technology for GSTM1 deletion polymorphism genotyping.

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Published

2016-09-15

How to Cite

Beránek, M., Fiala, Z., Kremláček, J., Palička, V., & Borská, L. (2016). The Technology of Digital PCR for Analysis of The Deletion Polymorphism in the GSTM1 Gene. Chemické Listy, 110(9), 655–657. Retrieved from http://ww.w.chemicke-listy.cz/ojs3/index.php/chemicke-listy/article/view/168

Issue

Vol. 110 No. 9 (2016)

Section

Articles

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