Utilizing Automated Chip Electrophoresis for Study of Lactoferrin and Matrix Metalloproteinases

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  • O. Zítka Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University, Brno,
  • S. Křížková Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University, Brno,
  • V. Adam Department of Animal Nutrition and Forage Production, Faculty of Agronomy, Mendel University, Brno,
  • A. Horna Radanal Ltd., Pardubice,
  • J. Kukačka Department of Clinical Biochemistry and Pathobiochemistry, 2nd Faculty of Medicine, Charles University, Prague,
  • R. Průša Department of Clinical Biochemistry and Pathobiochemistry, 2nd Faculty of Medicine, Charles University, Prague,
  • V. Žížková Research Institute for Knitting, Brno
  • R. Kizek Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University, Brno,

Abstrakt

Automated chip capillary electrophoresis was used in detection and isolation of lactoferrin and a human matrix metalloproteinase (MMP-9) as well as in investigation of interactions and cleavage of collagen with MMP-9. The method is a sensitive and simple technique superior to SDS-PAGE. It is useful in proteomic research.

Publikováno

15.04.2010

Jak citovat

Zítka, O., Křížková, S., Adam, V., Horna, A., Kukačka, J., Průša, R., Žížková, V., & Kizek, R. (2010). Utilizing Automated Chip Electrophoresis for Study of Lactoferrin and Matrix Metalloproteinases. Chemické Listy, 104(3). Získáno z http://ww.w.chemicke-listy.cz/ojs3/index.php/chemicke-listy/article/view/1341

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