High Accuracy of Analysis in Clinical Biochemistry. A Necessity or Not?

Authors

  • J. Arnoštová Department of Biochemistry and Microbiology, University of Chemistry and Technology, Prague
  • L. Arnoštová Central Laboratories, University of Chemistry and Technology, Prague
  • B. Holubová Department of Biochemistry and Microbiology, University of Chemistry and Technology, Prague

Keywords:

cortisol, Cushing's syndrome, immunochemical methods, diagnostics, clinical chemistry

Abstract

Methods for determination of biocompounds in biological samples, mainly molecules of steroid nature, have always been a well-discussed issue. This review is focused on the recent trend in clinical biochemistry, namely the use of methods that provide extreme accuracy for both research and clinical practice, such as liquid chromatography – mass spectroscopy/mass spectroscopy. These highly sophisticated methods may seem to have some advantages over the traditionally employed immunochemical methods (usually various types of enzyme immunoassay, mostly different modes of enzyme-linked immunosorbent assay). However, the former is significantly more costly, more time-consuming (as taken from the pre-analytic phase to verified results), and more demanding with respect to the qualification of the personnel. Thus, this review shows that, in many cases, these drawbacks cannot be compensated for by the benefits.

Published

2020-05-15

How to Cite

Arnoštová, J., Arnoštová, L., & Holubová, B. (2020). High Accuracy of Analysis in Clinical Biochemistry. A Necessity or Not?. Chemické Listy, 114(5), 349–354. Retrieved from http://ww.w.chemicke-listy.cz/ojs3/index.php/chemicke-listy/article/view/3603

Issue

Section

Articles